The kingdom fungi contains heterogeneous organisms, with over 100,000 described species (Kirk P.M, 2008), however, as expected Hawksworth (2001) the number of fungal species can be up to 1,500,000 species. Fungi are very meaningful in practice, many species make food: Volvariella, Auricularia, Pleurotus; medicinal products: Ganoderma luc, Trametes versicolor, Cordyceps etc applied in pharmaceutical technology. In science, many species (Lentinus tigrinus, Schizophyllum commune) is the subject of studies on physiology, biochemistry and genetics. Besides, there are many species of fungi that harm plants and animals, some poisonous fungus cause coma and death for human. Researches on macrofungi in Central Vietnam have been mentioned such as Patouillard, N. (1923, 1928), Joly P. (1968), Ngo A. (2003), Dörfelt, H., Trinh T.K, Berg, A. (2004) etc, However, fungus research is still limited, so collecting and classifying fungus in the Central and Highlands has significant scientific and practical significance. In Ngoc Linh mountain, Quang Nam province, not has studied the macro fungi. Therefore, “Research on the macro fungi Myxomycota, Ascomycota, Basidiomycota in Ngoc Linh mountain, Quang Nam province” is a necessary to identify and contribute to the protection of the diversity of species composition, the rational use of resources, the protection of rare genetic resources for the Vietnam mushroom complex.